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A – D thermodynamic interactions between peptides and the SCNN1α receptor; E – H thermodynamic interactions between peptides and the SCNN1β receptor; I – L thermodynamic interactions between peptides and the SCNN1γ receptor.

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A – D thermodynamic interactions between peptides and the SCNN1α receptor; E – H thermodynamic interactions between peptides and the SCNN1β receptor; I – L thermodynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Thermodynamic interaction results of salty peptide with ENaC taste receptor

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Thermodynamic interaction results of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

A Dynamic interactions between peptides and the SCNN1α receptor; B dynamic interactions between peptides and the SCNN1β receptor; C dynamic interactions between peptides and the SCNN1γ receptor.

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A Dynamic interactions between peptides and the SCNN1α receptor; B dynamic interactions between peptides and the SCNN1β receptor; C dynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Dynamic interactions result of salty peptide with ENaC taste receptor

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Dynamic interactions result of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Sequence information of ENaC taste receptor transmembrane structure

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Sequence information of ENaC taste receptor transmembrane structure

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques: Sequencing

A – D thermodynamic interactions between peptides and the SCNN1α receptor; E – H thermodynamic interactions between peptides and the SCNN1β receptor; I – L thermodynamic interactions between peptides and the SCNN1γ receptor.

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A – D thermodynamic interactions between peptides and the SCNN1α receptor; E – H thermodynamic interactions between peptides and the SCNN1β receptor; I – L thermodynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Thermodynamic interaction results of salty peptide with ENaC taste receptor

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Thermodynamic interaction results of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

A Dynamic interactions between peptides and the SCNN1α receptor; B dynamic interactions between peptides and the SCNN1β receptor; C dynamic interactions between peptides and the SCNN1γ receptor.

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A Dynamic interactions between peptides and the SCNN1α receptor; B dynamic interactions between peptides and the SCNN1β receptor; C dynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Dynamic interactions result of salty peptide with ENaC taste receptor

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Dynamic interactions result of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Sequence information of ENaC taste receptor transmembrane structure

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Sequence information of ENaC taste receptor transmembrane structure

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques: Sequencing